Part:BBa_K3237008
His-mRFP1-proinsulin-CPP with furin cut sites codon optimized for C. reinhardtii
This part was designed for targeted delivery of insulin into the blood stream from the intestinal wall. The Cell penetrating peptide (a protein transduction domain) has been found to increase the intestinal absorption of insulin [1] and is therefore able to increase insulin uptake from the GI tract and into the blood stream. This Cell penetrating peptide was fused onto the C-terminus of proinsulin with a furin cut site in between. The proinsulin itself was modified by the removal of original protease sites and replaced by furin cut sites as proposed originally by Tsingua2014. The proinsulin-CPP are attached to A his-mRFP1 coding sequence with linkers and furin cut sites [2] to remove the RFP after expression. In summary, this part expresses the His-mRFP1-prinsulin-CPP construct. After expression, the CPP and his-mRFP1 peptides are removed by the action of furin protease which also processes the insulin to an active form. This will only occur in vitro or after the construct passes through the intestinal wall. This part is codon optimized for chlamydomonas reinhardtii and has a complementary part codon optimized for E.coli BBa_K3237009.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 363
Illegal XhoI site found at 904 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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